Splicing up Pluripotency

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Splicing up Pluripotency

In this issue of Cell, Gabut and colleagues (2011) identify a new splice variant of FOXP1 that directly regulates the expression of pluripotency genes. It endows human embryonic stem cells with their pluripotent nature and is required for the reprogramming of somatic cells to induced pluripotent stem cells.

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Let's sp(l)ice up pluripotency!

mRNA-splicing presents an important regulatory step that contributes to the functional repertoire of all cell types and tissues. Profound changes in alternative splicing have also been revealed during embryonic stem cell (ESC) differentiation. Based on a genome-wide screen for pluripotency regulators, Ng and colleagues now identify SON as a new splicing regulator in the maintenance of human ESC...

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Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation.

Two major goals of regenerative medicine are to reproducibly transform adult somatic cells into a pluripotent state and to control their differentiation into specific cell fates. Progress toward these goals would be greatly helped by obtaining a complete picture of the RNA isoforms produced by these cells due to alternative splicing (AS) and alternative promoter selection (APS). To investigate ...

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An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming

Alternative splicing (AS) is a key process underlying the expansion of proteomic diversity and the regulation of gene expression. Here, we identify an evolutionarily conserved embryonic stem cell (ESC)-specific AS event that changes the DNA-binding preference of the forkhead family transcription factor FOXP1. We show that the ESC-specific isoform of FOXP1 stimulates the expression of transcript...

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Long non-coding RNA SOX2OT: expression signature, splicing patterns, and emerging roles in pluripotency and tumorigenesis

SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA which harbors one of the major regulators of pluripotency, SOX2 gene, in its intronic region. SOX2OT gene is mapped to human chromosome 3q26.3 (Chr3q26.3) locus and is extended in a high conserved region of over 700 kb. Little is known about the exact role of SOX2OT; however, recent studies have demonstrated a positive role for it in...

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ژورنال

عنوان ژورنال: Cell

سال: 2011

ISSN: 0092-8674

DOI: 10.1016/j.cell.2011.09.004